MyBioSource.com's Cofilin Antibody is a Rabbit Polyclonal antibody. This antibody has been shown to work in applications such as: ELISA, and Western Blot.
Description
Cofilin is a widely-distributed, intracellular, actin binding 20 kDa protein which is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus and is also involved in the regulation of actin assembly in vivo throughout muscle and non-muscle cells. Cofilin is a small phosphoinositide sensitive, actin binding protein capable of depolymerizing actin filaments in vitro. Cofilin, Destrin and other actyin depolymerizing factors bind stoichiometrically to monomeric G-actin and to actin protomers in filaments in a pH-dependent, Ca2+ independent manner. Numerous cofilin homologues are characterized in various organisms including mammals (1).Cofilin intercalates between longitudinally associated actin monomers within the filament and distorts its helical twist. Under certain conditions, it fragments the filaments and accelerates actin subunit dissociation from their 'pointed' (minus) ends. Cofilin is ubiquitous in tissues of eukaryotes and is abundant in neuronal tissues. It is essential for viability and is important for many cellular processes involving actin remodeling such as motility at the leading edge of cells, polarized cell growth, endocytosis, phagocytosis, cellular activation, cytokinesis, and pathogen intracellular motility.
Cofilin is ubiquitously expressed in eukaryotic cells where it binds to Actin, regulating the rapid cycling of Actin assembly and disassembly, essential for cellular viability. Cofilin 1, also known as Cofilin, non-muscle isoform (NM-CF), is a low molecular weight protein that binds to filamentous F-Actin by bridging two longitudinally-associated Actin subunits, changing the F-Actin filament twist. This process is allowed by the dephosphorylation of Cofilin Ser 3 by factors like opsonized zymosan. Cofilin 2, also known as Cofilin, muscle isoform (M-CF), exists as two alternatively spliced isoforms. One isoform is known as CFL2a and is expressed in heart and skeletal muscle. The other isoform is known as CFL2b and is expressed ubiquitously (2). Cofilin and actin-depolymerization factor (ADF) are members of a family of essential conserved small actin-binding proteins that play pivotal roles in cytokinesis, endocytosis, embryonic development, stress response, and tissue regeneration. In response to stimuli, cofilin promotes the regeneration of actin filaments by severing preexisting filaments (3). Cofilin gene maps to chromosome 11q13 and encodes a 166 amino acid (19-20kDa) protein.
Cofilin-selective antibodies were generated against a peptide taken from the human protein. The Cofilin-selective antibodies are affinity purified on an immobilized antigen based affinity matrix, the isolated antibodies were then stabilized in antibody stabilization buffer for long-term storage. The Cofilin-selective antibodies are fully characterized for applications in western blotting and ELISA at the recommended dilutions